Method for producing linear DNA fragments for the

专利名称：Method for producing linear DNA fragments

for the in vitro expression of proteins

发明人：JANY, Cordula, Dr.,Buchberger, Bernd,

Dr.,Watzele, Manfred, Dr.,Mutter, Wolfgang,Dr.,Roeder, Albert, Dr.,Wessner, Stephanie

申请号：EP02026225.9申请日：20021126公开号：EP1316616B1公开日：20150128

摘要：The present invention concerns a method for producing linear DNA fragmentsfor the expression of proteins in which a linear DNA fragment which contains controlelements necessary for expression and the protein-coding gene which has

complementary regions to the two ends of the linear DNA fragment at both ends, areamplified together using a primer pair which binds upstream and downstream of theexpression control regions on the linear DNA fragment, characterized in that the linearDNA fragment can be produced by linearizing an expression vector. Furthermore amethod is disclosed which concerns the preparation of the protein-coding gene the endsof which have regions which overlap the linear DNA fragment. The present invention alsoencompasses the linear DNA fragments according to the invention for the expression ofproteins, the use of these fragments, kits containing the linear DNA fragments accordingto the invention for the expression of proteins and methods for the expression ofproteins starting from the linear DNA fragments according to the invention.

申请人：ROCHE DIAGNOSTICS GMBH,HOFFMANN LA ROCHE

地址：DE,CH

国籍：DE,CH

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