Dianova DIA-303说明书

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Dianova DIA-303说明书

世界实验材料供应商Dianova正式授权上海起发为其中国代理，Dianova在一直是行业的 ,一直为广大科研客户提供zui为的产品和服务,上海起发一直秉承为中国科研客户带来的产品，的服务，签约Dianova就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

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Dianova公司为德国的病理免疫组化产品供应商，具备的抗备工艺及严格的质控系统，产品在上享有良好的声誉。

克隆号为的IDH1（isocitrate dehydrogenase 1, 异柠檬酸盐脱氢酶1）为Dianova公司近期推出的产品之一，此抗体可特异性的检测脑组织肿瘤中IDH1基因的R132H点突变。IDH1基因的第132密码子的杂合突变是和III级神经胶质瘤（根据WHO的肿瘤学分类）的频发事件。在大约70％的星形胶质细胞瘤和少突神经胶质细胞瘤可发现IDH1基因的R132H点突变。

基因R132H突变在部分脑肿瘤中的频发现象使克隆号为H09的IDH1抗体可的用于此类肿瘤的鉴别诊断中：如从原发性胶质母细胞瘤中区分出间变性星形细胞瘤、从毛细胞型星形细胞瘤或室管膜（细胞）瘤中区分出弥漫性星形细胞瘤（WHO分类II级），也可以用于具有IDH1突变的肿瘤细胞浸润前沿的与反应性神经胶质增多症的区分。因此，由于其高灵敏性和高特异性，此抗体在脑部肿瘤的分类分级中以及在检测单一或少量浸润肿瘤细胞时是非常有用的。

DIA-303 – Anti-CD3 e (Ms) aus Ratte (Klon: HH3E) für Paraffingewebe aus Maus – 500 μl

Art.-Nr. DIA-303

Spezifit?t CD3 e

Spezies-Reaktivit?t Maus

Wirtsspezies Ratte

Isotyp IgG1/k

Klon HH3E

Klonalit?t (Mono-/Polyklonal) monoklonal

Anwendung Immunhistochemie (IHC)

Immunhistochemie (Paraffingewebe)

Gefrierschnitte (IHC-F)

Western Blot

Konjugation unkonjugiert

Verdünnung Histo-/Zytochemie 1:100

Format Lyophilisat

gereinigter Antik?rper (aus Kulturüberstand)

Produktlinie / Thema CD-Marker

Immunhistochemie

Zweckbestimmung nur für Forschungszwecke

Suchcode DIA303M

DIA303

Hersler / Marke dianova

Reactivity:

Antibodyclone HH3Ehas been validated specifically for the detection of murineCD3in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). It detects aconserved epitope on the CD3 epsilon chainin a broad variety of species. CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used asT cell marker.

Cluster of differentiation 3 (CD3) is composed of four distinct polypeptide chains CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta, that form a multimeric protein complex. The CD3 complex associates non-covalently with the T cell receptor (TCR) and serves as a T cell co-receptor. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal trans-duction molecules. The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into in-tracellular signals that elicit a change in the cell from a dormant to an activated state. During T cell maturation the expression of CD3 migrates from the cytoplasm of pro-thymocytes to the cell-membrane of thymocytes. The specific appearance at all stages of T cell development make CD3 an ideal marker for normal T cells and T cell neoplasms (lymphomas, leukemias). Moreover, CD3 is a usefull immunohistochemical marker for T cells in tissue sections.

In a clinical setting in humans, CD3 serves as an important T cell marker for the classification of malignant lymphomas and leu-kemias. It can also be used to detect T cells in coeliac disease, lymphocytic and collagenous colitis. An anti-CD3 epsilon anti-body (Okt3) has been clinically approved for the induction of immunosuppression in organ transplantation. In animal studies anti-CD3 antibodies can induce tolerance to allografts.

Immunohistochemistry of mouse CD3e (TCRE) in formalin-fixed paraffin-embedded tissue sections

The monoclonal antibody clone HH3E specifically stains mouse tissue sections by IHC-FFPE: Lymph nodes (A,B), spleen (C) and kidney (D).

All sections were stained by an indirect alkaline phosphatase method according to standard procedures with antigen retrieval by high-temperature heating in citrate buffer and counterstaining with Haematoxylin.

Related References:

Leon F. Flow cytometry of intestinal intraepithelial lymphocytes in celiac disease. Journal of Immunological Methods 363: 177-186, 2011

Smith-Garvin JE et al.. T cell activation. Annu Rev Immunol. 27:591-619, 2009

Sapp H et al.The terminal ileum is affected in patients with lymphocytic or collagenous colitis. Am J Surg Pathol. 26(11):1484-1492, 2002

Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clon-ality by polymerase chain reaction. Vet Immunol Immunopathol. 69:145-1, 1999

Mosnier et al. Lymphocytic and collagenous colitis: an immunohistochemical study. Am J Gastroenterol. 91(4):709-713, 1996

Chetty R, Gatter K. CD3: Structure, function, and role of immunostaining in clinical practice. The Journal of Pathology 173(4): 303–307, 1994

Salvadori S et al. Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors. J Immunol. 153(11):5176-5182, 1994

Nicolls MR et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody. Transplantation 55(3):459-468, 1993