RNA sequence-specific mediators of RNA interferenc

专利名称：RNA sequence-specific mediators of RNA

interference

发明人：Thomas Tuschl,Phillip D. Zamore,Phillip A.

Sharp,David P. Bartel

申请号：US11474930申请日：20060626

公开号：US20070003962A1公开日：20070104

专利附图：

摘要：The present invention relates to a Drosophila in vitro system which was used todemonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length.

Furthermore, when these 21-23 nt fragments are purified and added back to Drosophilaextracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23nt fragments are the sequence-specific mediators of RNA degradation. A molecularsignal, which may be their specific length, must be present in these 21-23 nt fragments torecruit cellular factors involved in RNAi. This present invention encompasses these 21-23nt fragments and their use for specifically inactivating gene function. The use of thesefragments (or chemically synthesized oligonucleotides of the same or similar nature)enables the targeting of specific mRNAs for degradation in mammalian cells, where theuse of long dsRNAs to elicit RNAi is usually not practical, presumably because of thedeleterious effects of the interferon response. This specific targeting of a particular genefunction is useful in functional genomic and therapeutic applications.

申请人：Thomas Tuschl,Phillip D. Zamore,Phillip A. Sharp,David P. Bartel

地址：New York NY US,Northborough MA US,Newton MA US,Brookline MA US

国籍：US,US,US,US

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